Nogara Leonardo, et al.
Proceedings of the National Academy of Sciences, 2016
Abstract
We identify a target for treating obesity and type 2 diabetes, the consumption of calories by an increase in the metabolic rate of resting skeletal muscle. The metabolic rate of skeletal muscle can be increased by shifting myosin heads from the super-relaxed state (SRX), with a low ATPase activity, to a disordered relaxed state (DRX), with a higher ATPase activity. The shift of myosin heads was detected by a change in fluorescent intensity of a probe attached to the myosin regulatory light chain in skinned skeletal fibers, allowing us to perform a high-throughput screen of 2,128 compounds. The screen identified one compound, which destabilized the super-relaxed state, piperine (the main alkaloid component of black pepper). Destabilization of the SRX by piperine was confirmed by single-nucleotide turnover measurements. The effect was only observed in fast twitch skeletal fibers and not in slow twitch fibers or cardiac tissues. Piperine increased ATPase activity of skinned relaxed fibers by 66 ± 15%. The Kd was ∼2 µM. Piperine had little effect on the mechanics of either fully active or resting muscle fibers. Previous work has shown that piperine can mitigate both obesity and type 2 diabetes in rodent models of these conditions. We propose that the increase in resting muscle metabolism contributes to these positive effects. The results described here show that up-regulation of resting muscle metabolism could treat obesity and type 2 diabetes and that piperine would provide a useful lead compound for the development of these therapies.
Keywords
fluorescence; myosin; obesity; skeletal muscle; super-relaxed state
Free PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5135373/.
| PMID: | 27799519 |
|---|---|
| PMCID (Free PMC Article): | PMC5135373 |
| Category: | Weight Loss |
The best supplements with Piperine in Weight Loss category:
- Testo Prime - TestoPrime is an all-natural testosterone support that can help any man take control of his youthful vitality with a flood of new and natural testosterone. It contains among others: Piperine.
- Keto Actives - Keto Actives is a modern dietary supplement that helps you lose weight even when it comes to the most difficult parts of your body. It contains among others: Piperine.
- PhenQ - PhenQ is a powerful new dieting formula combining multiple weight loss benefits to help you get the slim, sexy body you’ve always wanted. It contains among others: Piperine.
- Fast Burn Extreme - Fast Burn Extreme is a unique multi-component fat reducer. It contains among others: Piperine.
- Silvets - Silvets constitute modern tablets that facilitate weight loss dedicated to those persons that wish to lose weight and stay in a good mood. It contains among others: Piperine.
- Nuratrim - Nuratrim formula works by helping boost energy levels, digestion and metabolism. It contains among others: Piperine.
- Piperinox - Are you tired of constantly trying to lose weight? Choose Piperinox and enjoy a beautiful figure! Piperinox is a recommendable dietary supplement supporting the weight loss process. It contains among others: Piperine.
Previous article
Anticancer Properties of Capsaicin Against Human Cancer.
Next article
Protective effects of taurine on human hair follicle grown in vitro.