Annona Muricata Linn. Leaf as a Source of Antioxidant Compounds With in Vitro Antidiabetic and Inhibitory Potential Against α-Amylase, α-Glucosidase, Lipase, Non-Enzymatic Glycation and Lipid Peroxidation.

Justino Allisson Benatti, et al.
Biomedicine & Pharmacotherapy, 2018

Abstract

Annona muricata leaves are used in traditional medicine to manage diabetes mellitus and its complications. The aim of this study was to evaluate the potential in vitro antidiabetic properties of Annona muricata leaf by identifying its main phytochemical constituents and characterizing the phenolic-enriched fractions for their in vitro antioxidant capacity and inhibitory activities against glycoside and lipid hydrolases, advanced glycation end-product formation and lipid peroxidation. Ethanol extract of A. muricata leaf was subjected to a liquid-liquid partitioning and its fractions were used in enzymatic assays to evaluate their inhibitory potential against α-amylase, α-glucosidase and lipase, as well as their antioxidant (DPPH, ORAC, FRAP and Fe²⁺-ascorbate-induced lipid peroxidation assays) and anti-glycation (BSA-fructose, BSA-methylglyoxal and arginine-methylglyoxal models) capacities. In addition, identification of the main bioactive compounds of A. muricata leaf by HPLC-ESI-MS/MS analysis was carried out. Ethyl acetate (EtOAc) and n-butanol (BuOH) fractions showed, respectively, antioxidant properties (ORAC 3964 ± 53 and 2707 ± 519 μmol trolox eq g^-1, FRAP 705 ± 35 and 289 ± 18 μmol trolox eq g^-1, and DPPH IC₅₀ 4.3 ± 0.7 and 9.3 ± 0.8 μg mL^-1) and capacity to reduce liver lipid peroxidation (p < .01). Also, EtOAc and BuOH, respectively, inhibited glycation in BSA-fructose (IC₅₀ 45.7 ± 13.5 and 61.9 ± 18.2 μg mL^-1), BSA-methylglyoxal (IC₅₀ 166.1 ± 21.6 and 413.2 ± 49.5 μg mL^-1) and arginine-methylglyoxal (IC₅₀ 437.9 ± 89.0 and 1191.0 ± 199.0 μg mL^-1) assays, α-amylase (IC₅₀ 9.2 ± 2.3 and 6.1 ± 1.6 μg mL^-1), α-glucosidase (IC₅₀ 413.1 ± 121.1 and 817.4 ± 87.9 μg mL^-1) and lipase (IC₅₀ 74.2 ± 30.1 and 120.3 ± 50.5 μg.mL^-1), and presented lower cytotoxicity, when compared to the other fractions and crude extract. Various biomolecules known as potent antioxidants were identified in these fractions, such as chlorogenic and caffeic acids, procyanidins B2 and C1, (epi)catechin, quercetin, quercetin-hexosides and kaempferol. This study presents new biological activities not yet described for A. muricata, which contributes to the understanding of the potential effectiveness in the use of the A. muricata leaf, especially its polyphenols-enriched fractions, for the management of diabetes mellitus and its complications. eywords: Annonaceae; Glycation; Natural products; Phenolic compounds; Soursop; Starch hydrolase.

Copyright © 2018 Elsevier Masson SAS. All rights reserved.

Previous article

Immunomodulatory Efficacy of Standardized Annona muricata (Graviola) Leaf Extract via Activation of Mitogen-Activated Protein Kinase Pathways in RAW 264.7 Macrophages.

Next article

Bioefficacy of Graviola Leaf Extracts in Scavenging Free Radicals and Upregulating Antioxidant Genes.