Songsiripradubboon Siriporn, et al.
Journal of endodontics, 2017
Abstract
Introduction
This study investigated the effects of acemannan, a polysaccharide from Aloe vera, on human deciduous pulp cells in vitro and the response after vital pulp therapy in dog deciduous teeth.
Methods
Human primary dental pulpal cells were treated with acemannan in vitro and evaluated for proliferation, alkaline phosphatase activity, type I collagen, bone morphogenetic protein (BMP-2), BMP-4, vascular endothelial growth factor, and dentin sialoprotein expression and mineralization. Osteogenesis-related gene expression was analyzed by complementary DNA microarray. Pulpal inflammation was induced in dog teeth for 14 days. The inflamed pulp was removed, retaining the healthy pulp. The teeth were randomly divided into 3 treatment groups: acemannan, mineral trioxide aggregate, and formocresol. Sixty days later, the teeth were extracted and evaluated histopathologically.
Results
Acemannan significantly increased pulp cell proliferation, alkaline phosphatase, type I collagen, BMP-2, BMP-4, vascular endothelial growth factor, and dentin sialoprotein expression and mineralization approximately 1.4-, 1.6-, 1.6-, 5.5-, 2.6-, 3.8-, 1.8-, and 4.8-fold, respectively, compared with control. In vivo, partial pulpotomy treatment using acemannan generated outcomes similar to mineral trioxide aggregate treatment, resulting in mineralized bridge formation with normal pulp tissue without inflammation or pulp necrosis. In contrast, the formocresol group demonstrated pulp inflammation without mineralized bridge formation.
Conclusions
Acemannan is biocompatible with the dental pulp. Furthermore, acemannan stimulated dentin regeneration in teeth with reversible pulpitis.
Keywords
Acemannan; MTA; animal model; dentin regeneration; microarray; vital pulp therapy.
Copyright © 2017 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
| PMID: | 28477996 |
|---|---|
| DOI: | 10.1016/j.joen.2017.01.037 |
| Category: | Joint / Bone Health |
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Background/purpose
Acemannan, a β-(1-4)-acetylated polymannose extracted from Aloe vera gel, has been proposed as biomaterial for bone regeneration... Acemannan is an effective bioactive agent for bone regeneration, enhancing bone growth as assayed in two- and three-dimensions. )