González-Castejón Marta, et al.
Phytotherapy research, 2014
Abstract
In this in vitro study, we have investigated the ability of Taraxacum officinale (dandelion) to inhibit adipocyte differentiation and lipogenesis in 3T3-L1 preadipocytes. HPLC analysis of the three plant extracts used in this study-leaf and root extracts and a commercial root powder-identified caffeic and chlorogenic acids as the main phenolic constituents. Oil Red O staining and triglyceride levels analysis showed decreased lipid and triglyceride accumulation, respectively. Cytotoxicity was assessed with the MTT assay showing non-toxic effect among the concentrations tested. DNA microarray analysis showed that the extracts regulated the expression of a number of genes and long non-coding RNAs that play a major role in the control of adipogenesis. Taken together, our results indicate that the dandelion extracts used in this study may play a significant role during adipogenesis and lipid metabolism, and thus, supporting their therapeutic interest as potential candidates for the treatment of obesity.
Keywords
3 T3-L1 cells; adipogenesis; dandelion; differentiation; long noncoding RNAs; obesity; phytochemicals.
Copyright © 2013 John Wiley & Sons, Ltd.
PMID: | 23956107 |
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DOI: | 10.1002/ptr.5059 |
Category: | Weight Loss |
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