Ilham Rizky, et al.
Open Access Macedonian Journal of Medical Sciences, 2019
Abstract
Background
Dengue haemorrhagic fever (DHF) is a disease found in most tropical and subtropical regions of the world, including Indonesia. One of the problems of vector control programs is insecticides resistance to Aedes spp.
Aim
The objective of this study is to determine the effectiveness of an alternative larvacide using papaya leaves (Carica papaya L).
Methods
To obtain an ethanolic extract of C. papaya leaf (EECP), the dried of C. papaya leaf was macerated with ethanol 70%. Phytochemical compounds were screened qualitatively. Twenty-five larvae were entered into each cup that had been mixed with five concentrations of EECP i.e., EECPI (100-), EECPII (150-), EECPIII (200-), EECPIV (250), EECPV (300 ppm), 1% of Temephos (T), and water (A). Alkaloid carpain, saponin, flavonoid, tannin, glycosides and triterpenoid/steroid were traced in EECP. The mortality of larvae at 180, 360, 1440 and 2880 minutes were observed. The lethal concentration (LC50) and lethal time (LT50) were measured. Probit analysis was used to determine the concentration of killing larvae.
Results
The mortality of larvae was found at 360 minutes only in EECPV. Then after 1440 minutes, all extracts shown the increasing of larvae mortality. LC50 and LT50 values were 215,96 ppm and 2,369 minutes of each.
Conclusion
EECP has larvicidal activity to Aedes spp.
Keywords
Aedes spp; Carica papaya L; Dengue; Larvacide.
Copyright: © 2019 Rizky Ilham, Aznan Lelo, Urip Harahap, Tri Widyawati, Lambok Siahaan.
PMID: | 32002060 |
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PMCID (Free PMC Article): | PMC6980815 |
DOI: | 10.3889/oamjms.2019.432 |
Category: | Cleansing |
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