Chen Gang, et al.
Artificial cells, nanomedicine, and biotechnology, 2016
Abstract
Objective
To investigated the antioxidant protection effects of vitamin C (Vc) on the hemoglobin-based oxygen carriers (HBOCs) solution.
Methods
Three influential variables were studied including the molar ratio of Vc and hemoglobin, pH and temperature. The HBOCs molecules were characterized through spectral analysis, circular dichroism spectra, the oxygen binding curves and the molecular weights.
Results
The optimized conditions were molar ratios of above 3, pH of 7.5-8.0 and temperature of 4 °C. HBOCs characterization showed no differences before and after Vc addition.
Conclusion
Antioxidant effects in HBOCs solution could be presented by Vc, indicating the potential application of Vc in HBOCs development.
Keywords
antioxidant effects; hemoglobin-based oxygen carriers; methemoglobin; vitamin C.
PMID: | 26671172 |
---|---|
DOI: | 10.3109/21691401.2015.1111239 |
Category: | Antioxidants |
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