Antioxidant Effects of Vitamin C on Hemoglobin-Based Oxygen Carriers Derived From Human Cord Blood.

Chen Gang, et al.
Artificial cells, nanomedicine, and biotechnology, 2016

Abstract

Objective

To investigated the antioxidant protection effects of vitamin C (Vc) on the hemoglobin-based oxygen carriers (HBOCs) solution.

Methods

Three influential variables were studied including the molar ratio of Vc and hemoglobin, pH and temperature. The HBOCs molecules were characterized through spectral analysis, circular dichroism spectra, the oxygen binding curves and the molecular weights.

Results

The optimized conditions were molar ratios of above 3, pH of 7.5-8.0 and temperature of 4 °C. HBOCs characterization showed no differences before and after Vc addition.

Conclusion

Antioxidant effects in HBOCs solution could be presented by Vc, indicating the potential application of Vc in HBOCs development.

Keywords

antioxidant effects; hemoglobin-based oxygen carriers; methemoglobin; vitamin C.

PMID:26671172
DOI:10.3109/21691401.2015.1111239
Category:Antioxidants

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