Luteolin Isolated From the Flowers of Lonicera Japonica Suppresses Inflammatory Mediator Release by Blocking NF-kappaB and MAPKs Activation Pathways in HMC-1 Cells.

Kang Ok-Hwa, et al.
Molecules, 2010

Abstract

Luteolin (3',4',5,7-tetrahydroxylflavone) is a plant flavonoid and pharmacologically active agent that has been isolated from several plant species. In the present study, the effect of luteolin from the flowers of Lonicera japonica on phorbol 12-myristate 13-acetate (PMA) plus A23187-induced mast cell activation was examined. Luteolin significantly inhibited the induction of inflammatory cytokines such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-8, IL-6 and granulocyte-macrophage colony-stimulating factor (GM-CSF) by PMA plus A23187. Moreover, luteolin attenuated cyclooxygenase (COX)-2 expression and intracellular Ca2+ levels. In activated HMC-1 cells, the phosphorylation of extra-signal response kinase (ERK 1/2) and c-jun N-terminal Kinase (JNK 1/2), but not p38 mitogen-activated protein kinase (p38 MAPK) were decreased by treatment of the cells with luteolin. Luteolin inhibited PMA plus A23187-induced nuclear factor (NF)-kappaB activation, IkappaB degradation, and luciferase activity. Furthermore, luteolin suppressed the expression of TNF-alpha, IL-8, IL-6, GM-CSF, and COX-2 through a decrease in the intracellular Ca2+ levels, and also showed a suppression of the ERK 1/2, JNK 1/2, and NF-kappaB activation.

Conclusions

These results indicated that luteolin from the flowers of Lonicera japonica exerted a regulatory effect on mast cell-mediated inflammatory diseases, such as RA, allergy disease and IBD.

Articles similar to "Luteolin Isolated From the Flowers of Lonicera Japonica Suppresses Inflammatory Mediator Release by Blocking NF-kappaB and MAPKs Activation Pathways in HMC-1 Cells."

• The role of Lonicera Japonica in Allergy / Immune: Antibacterial Activity of 20 Kinds of Chinese Medicinal Materials for Helicobacter Pylori in Vitro. (Objective: To study the antibacterial activity of 20 Chinese medicinal materials for helicobacter pylori in vitro and the culture of hylicobacter pylori...)

Previous article

Effect of Lonicerae Flos Extracts on Reflux Esophagitis With Antioxidant Activity.

Next article

Berberine and Coptidis Rhizoma as Novel Antineoplastic Agents: A Review of Traditional Use and Biomedical Investigations.