Jiang Yunhe, et al.
Antiviral Research, 2020
Abstract
Enterovirus D68 (EV-D68) is a member of the Picornavirus family and a causative agent of respiratory diseases in children. The incidence of EV-D68 infection has increased worldwide in recent years. Thus far, there are no approved antiviral agents or vaccines for EV-D68. Here, we show that methyl-β-cyclodextrin (MβCD), a common drug that disrupts lipid rafts, specifically inhibits EV-D68 infection without producing significant cytotoxicity at virucidal concentrations. The addition of exogenous cholesterol attenuated the anti-EV-D68 activity of MβCD. MβCD treatment had a weak influence on the attachment of viral particles to the cell membrane but significantly inhibited EV-D68 entry into host cells. We demonstrated that EV-D68 facilitated the translocation of the viral receptor ICAM-5 to membrane rafts in infected cells. The colocalization of viral particles with ICAM-5 in lipid rafts was thoroughly abolished in cells after treatment with MβCD. Finally, we showed that MβCD inhibited the replication of isolated circulating EV-D68 strains. In summary, our results demonstrate that MβCD suppresses EV-D68 replication by perturbing the accumulation of virus particles and ICAM-5 in lipid rafts. This mechanism represents a promising strategy for drug development.
Keywords
Enterovirus D68; ICAM-5; Lipid rafts; MβCD.
Copyright © 2020 Elsevier B.V. All rights reserved.
Conflict of interest statement
Declaration of competing interest The authors declare that there is no conflict of interest.| PMID: | 32101770 |
|---|---|
| DOI: | 10.1016/j.antiviral.2020.104752 |
| Category: | Immune |
The best supplements with Andrographis Paniculata in Immune category:
- Andrographis Extract, 400 mg, 90 Veg Capsules (Now Foods) - Andrographis is an herb traditionally used by herbalists in China, India, and Southeast Asia. It contains among others: Andrographis Paniculata.
Articles similar to "Methyl-β-cyclodextrin Inhibits EV-D68 Virus Entry by Perturbing the Accumulation of Virus Particles and ICAM-5 in Lipid Rafts."
- The significance of Andrographis Paniculata for Immune: Andrographolide Prevents EV-D68 Replication by Inhibiting the Acidification of Virus-Containing Endocytic Vesicles. (Enterovirus D68 (EV-D68) has emerged as a significant respiratory pathogen that can cause severe respiratory disease and acute neurologic disease...)
- The impact of Andrographis Paniculata on Immune: Anti-inflammatory and Antimicrobial Effects of Heat-Clearing Chinese Herbs: A Current Review. (Inflammation is a normal immune response; but if the body's regulation of inflammation is dysfunctional, then it will have an adverse effect on the body...)
- The significance of Andrographis Paniculata for Immune: Immunostimulant Agents From Andrographis Paniculata. (EtOH extract and purified diterpene andrographolides of Andrographis paniculata (Acanthaceae) induced significant stimulation of antibody and delayed type hypersensitivity (DTH) response to sheep red blood cells (SRBC) in mice...)
- The role of Andrographis Paniculata in Immune: The Effect of Medicinal Plants Used in Chinese Folk Medicine on RANTES Secretion by Virus-Infected Human Epithelial Cells. (The accumulation of inflammatory cells in the infective sites has been reported to play a crucial role in the progression of chronic inflammation and multiple sclerosis after viral infection...)
- The impact of Andrographis Paniculata on Immune: Anti-dengue Activity of Andrographis paniculata Extracts and Quantification of Dengue Viral Inhibition by SYBR Green Reverse Transcription Polymerase Chain Reaction. ( Dengue virus is a leading cause of illness and death in the tropics and subtropics... Ethanolic extracts treated with dengue VLs also showed a significant changes which were reflected in RT PCR assay. )
Previous article
Next article
Anti-inflammatory and Antimicrobial Effects of Heat-Clearing Chinese Herbs: A Current Review.